Antiviral activity of oleandrin and a defined extract of Nerium oleander against SARS-CoV-2
ZitatHere, we present in vitro evidence for significant inhibition of SARS-CoV-2 by oleandrin and a defined extract of N. oleander (designated as PBI-06150). Using Vero cells, we found that prophylactic (pre-infection) oleandrin (as either the pure compound or as the active principal ingredient in PBI-06150) administration at concentrations as low as 0.05 µg/ml exhibited potent antiviral activity against SARS-CoV-2, with an 800-fold reduction in virus production, and a 0.1 µg/ml concentration resulted in a greater than 3000-fold reduction in infectious virus production. The half maximal effective concentration (EC50) values were 11.98 ng/ml when virus output was measured at 24 h post-infection, and 7.07 ng/ml measured at 48 h post-infection. Therapeutic (post-infection) treatment up to 24 h after SARS-CoV-2 infection of Vero cells also reduced viral titers, with 0.1 µg/ml and 0.05 µg/ml concentrations causing greater than 100-fold reduction as measured at 48 h, and the 0.05 µg/ml concentration resulting in a 78-fold reduction. Concentrations of oleandrin up to 10 µg/ml were well tolerated in Vero cells. We also present in vivo evidence of the safety and efficacy of defined N. oleander extract (PBI-06150), which was administered to golden Syrian hamsters in a preparation containing as high as 130 µg/ml of oleandrin. In comparison to administration of control vehicle, PBI-06150 provided a statistically significant reduction of the viral titer in the nasal turbinates (nasal conchae). The potent prophylactic and therapeutic antiviral activities demonstrated here, together with initial evidence of its safety and efficacy in a relevant hamster model of COVID-19, support the further development of oleandrin and/or defined extracts containing this molecule for the treatment of SARS-CoV-2 and associated COVID-19 disease and potentially also for reduction of virus spread by persons diagnosed early after infection.
Kommentar: "Conflicts of interest statement" beachten. Kleiner Wermutstropfen außerdem: Den besten Effekt hatte es vor der Infektion (prophylaktisch).
Antiviral Effects of Oleandrin
ZitatGiven the fact that oleandrin affects viral infectivity in a unique manner highlights the possibility that it represents a novel strategy to block a virus’s ability to infect new cells and could serve as a viable therapeutic option against diseases such as COVID-19. In addition, having the ability to cross the blood–brain barrier and therein activate Nrf-2 and antioxidant genes may also present a new strategy against the prolonged secondary effects of COVID-19 since it has only recently been recognized that SARS-CoV-2 adversely affects neuronal and brain tissues2,89. The preventative as well as therapeutic potential of a new method to broadly target enveloped viruses should be given serious consideration in the era of the current coronavirus pandemic.
Kommentar: Hier gibt es ebenfalls eine "Disclosure".
Differential Activities of the Botanical Extract PBI-05204 and Oleandrin on Innate Immune Functions under Viral Challenge Versus Inflammatory Culture Conditions
ZitatThe Nerium oleander extract PBI 05204 (PBI) and its cardiac glycoside constituent oleandrin have direct anti-viral properties. Their effect on the immune system, however, is largely unknown. (...) The results show that PBI and oleandrin directly activate innate immune cells, enhance anti-viral immune responses through NK cell activation and IFN-γ levels, and modulate immune responses under inflamed conditions.
Targeting stress induction of GRP78 by cardiac glycoside oleandrin dually suppresses cancer and COVID-19
ZitatOur findings validate GRP78 as a target of OLN anti-cancer and anti-viral activities. These proof-of-principle studies support further investigation of OLN as a readily accessible compound to dually combat cancer and COVID-19.
Folgende Probleme bleiben: Sichere Dosierung und Sourcing (wo bekommt man reines Oleandrin her?). Da Überdosierung tödlich sein kann, ist hier natürlich besondere Vorsicht geboten. Der Thread soll nur informieren und niemanden zu potentiell gefährlichen Selbstexperimenten anregen.
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Oleandrin
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05.10.2024 23:28
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